Separation and screening of Acetobacter producing alcohol dehydrogenase

Abstract

In mammals, alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) constitute the main oxidation channels of ethanol metabolism, which are the main limiting factors of ethanol metabolism in the body. ADH mainly catalyzes the production of acetaldehyde from ethanol and is the first step in the oxidized process of ethanol in various organs and tissues of humans and animals. Therefore, it is of great significance in relieving alcoholism and preventing liver damage caused by ethanol. Alcohol dehydrogenase has been found in animals, plants, microorganisms, eukaryotes, and prokaryotic bacteria, which is widely presented in microorganisms. Modern research has proven that the brewing of vinegar is carried out by cell membranes of acetic acid bacteria binding enzymes that complete acetic acid fermentation through the oxidation of alcohol-by-alcohol dehydrogenase and the oxidation of acetaldehyde-by-acetaldehyde dehydrogenase. The fermentative ability of acetic acid bacteria is related to the activity of these two enzymes, especially the former. Therefore, isolating strains with high ADH production from vinegar is of great research which is significant for establishing a probiotic library for detoxifying alcohol and protecting the liver. This article enriched and screened well-known vinegar mash samples in China, and obtained Acetobacter D2 with ADH activity reaching 4.288×10－2U. Furthermore, its activity was improved to 8.254×10－2U through UV mutagenesis. The object of the work is Acetobacter D2 with ADH activity. The subject of the work is ADH activity of isolated from vinegar Acetobacter D2. The aim of the work is screen and isolate effective Acetobacter produced alcohol dehydrogenase. The tasks of the work are to isolate from vinegar ADH active strain of Acetobacter, obtain the ADH activity, and improve ADH activity with UV mutagenesis.